摘要
本研究探讨了保存温度和保存时间对牛体外受精胚胎存活率的影响。试验１测定第７，８ｄ的囊胚分别在４℃，２０℃和３９℃下保存（或培养）４～６ｈ，２４ｈ和４８ｈ后在体外培养的孵化率。在４℃下保存的胚胎的孵化率分别为７１．２％，５７．１％及２７．０％，显著低于对照组（８７．８％，Ｐ＜０．０１）。在２０℃和３９℃下保存（或培养）４～６ｈ不影响胚胎孵化率（分别为８４．９％，８５．９％），但保存２４ｈ和４８ｈ后，孵化率均显著降低（Ｐ＜０．０１）。试验２用ＰＲＩＤ阴道栓处理青年母牛，使其发情同期化，在发情开始后的第７ｄ，用非手术法给每头母牛移植２枚在２０℃下保存３～７ｈ或１８～２４ｈ的囊胚。移植保存３～７ｈ的胚胎获得的妊娠率、双胎率分别为７１．０％和５０．０％；移植保存１８～２４ｈ的胚胎的妊娠率显著降低（４１．５％），但双胎率（４１．２％）与保存３～７ｈ者无显著差异（Ｐ＞０．０５）。
The present experiments were undertaken to determine the effect of storage temperature and time on the survival of bovine blastocysts derived from in vitro fertilization and culture. Exp. 1, embryos were cultured in vitro after they were stored in modified PBS at 4℃, 20℃ or 39℃ for 4￣6 h, 24 h and 48 h. Storage at 4℃ significantly reduced the hatching rates (71. 2%, 57. 1 %, 27. 0% respectively after 4￣6 h, 24 h and 48 h) compared with that (87. 8% ) of the controls (P<0. 01). At 20℃ and 39℃, storage for 4￣6 h did not affect the viability of embryos (84. 9%, 85. 9% respectively), but storage for 24 h and 48 h significantly reduced the hatching rate (P<0.01).Exp. 2, 72 heifers synchronized by PRID treatment were nonsurgically transferred each with 2 blastocysts stored at 20℃ for 3￣7 h or 18￣24 h. Embryos stored for 3￣7 h yielded a high pregnancy rate (71.0% ) and twinning rate (50. 0% ). Storage of embryos for 18￣24 h resulted in a significantly lower pregnancy rate (41. 5%, P<0. 01), but did not affect the twinning rate (41. 2 % ).
关键词
牛
体外受精
保存温度
存活率
胚胎
bovine
blastocyst
in vitro fertilization
hatching rate
pregnancy rate
storage temperature
