摘要
本研究探讨了保存温度和保存时间对牛体外受精胚胎存活率的影响。试验1测定第7,8d的囊胚分别在4℃,20℃和39℃下保存(或培养)4~6h,24h和48h后在体外培养的孵化率。在4℃下保存的胚胎的孵化率分别为71.2%,57.1%及27.0%,显著低于对照组(87.8%,P<0.01)。在20℃和39℃下保存(或培养)4~6h不影响胚胎孵化率(分别为84.9%,85.9%),但保存24h和48h后,孵化率均显著降低(P<0.01)。试验2用PRID阴道栓处理青年母牛,使其发情同期化,在发情开始后的第7d,用非手术法给每头母牛移植2枚在20℃下保存3~7h或18~24h的囊胚。移植保存3~7h的胚胎获得的妊娠率、双胎率分别为71.0%和50.0%;移植保存18~24h的胚胎的妊娠率显著降低(41.5%),但双胎率(41.2%)与保存3~7h者无显著差异(P>0.05)。
The present experiments were undertaken to determine the effect of storage temperature and time on the survival of bovine blastocysts derived from in vitro fertilization and culture. Exp. 1, embryos were cultured in vitro after they were stored in modified PBS at 4℃, 20℃ or 39℃ for 4 ̄6 h, 24 h and 48 h. Storage at 4℃ significantly reduced the hatching rates (71. 2%, 57. 1 %, 27. 0% respectively after 4 ̄6 h, 24 h and 48 h) compared with that (87. 8% ) of the controls (P<0. 01). At 20℃ and 39℃, storage for 4 ̄6 h did not affect the viability of embryos (84. 9%, 85. 9% respectively), but storage for 24 h and 48 h significantly reduced the hatching rate (P<0.01).Exp. 2, 72 heifers synchronized by PRID treatment were nonsurgically transferred each with 2 blastocysts stored at 20℃ for 3 ̄7 h or 18 ̄24 h. Embryos stored for 3 ̄7 h yielded a high pregnancy rate (71.0% ) and twinning rate (50. 0% ). Storage of embryos for 18 ̄24 h resulted in a significantly lower pregnancy rate (41. 5%, P<0. 01), but did not affect the twinning rate (41. 2 % ).
关键词
牛
体外受精
保存温度
存活率
胚胎
bovine
blastocyst
in vitro fertilization
hatching rate
pregnancy rate
storage temperature