摘要
在１９９１～１９９３年间，应用国产和进口的促卵泡素（ＦＳＨ）及国产的孕马血清促性腺激素（ＰＭＳＧ）和人绒毛膜促性腺激素（ＨＣＧ），对１３６只母兔进行超数排卵处理试验。结果显示，国产的高活性ＦＳＨ对母兔有良好的超排作用。试验母兔中有８５％表现超排效应，平均每只母兔可回收到３１．１个卵，几乎相当于对照组（自然发情组，８．５个／只）的４倍。母兔对超排的反应表现出明显的季节性，春季时的处理效果极显著地优于秋季。其中ＦＳＨ组在春、秋季的回收卵数分别为３５．０个／只与１１．９个／只，差异十分显著（Ｐ＜０．００１）；ＰＭＳＧ组也表现类似效果。另外，注射ＨＣＧ后不同回收时间对卵回收数的影响也很大。在注射了ＨＣＧ１３．５ｈ后卵母细胞的回收数非常显著地比１３．５ｈ前高，两者几乎相差１倍（３６．０：１８．３）。同时，母兔左、右侧卵巢排卵数也表现出明显差异，不论是自然发情或人工超排，母兔右侧卵巢的排卵数均显著地高于左侧卵巢。
The effects of FSH (made in China or Sigma), PMSG and HCG on superovulation of 136 female rabbits during 1991￣1993 are reported. Results indicate that the high active FSH (made in China) has a very good superovulation effect on rabbits. Among the treated animals, 85 % responded to the treatment and an average of 31. 1 ova per rabbit were collected, which was nearly 4 times than that of the natural oestrous rabbits (8. 5).The superovulation response of rabbits is better in Spring than in Autumn. The average number of ova collected from FSH-treated rabbits in Spring (35. 0) was significantly greater than that in Autumn (11. 9, P<0. 001), and so was the case in PMSG-administrated rabbits. In addition, the ova collection time had a clear effect on the number of ova harvested. The number of ova collected more than 13. 5 h after HCG injection was nearly double of ova collected with in 13. 5 h (36. 0 vs 18. 3). Significantly more ova were ovulated from the right ovary than from the left one during both natural oestrous and superovulation.
关键词
兔
促性腺激素
超数排卵
排卵数
rabbits
gonadotrophin
superovulation
ovulation number
seasonal effect
ova collection time
