摘要
在1991~1993年间,应用国产和进口的促卵泡素(FSH)及国产的孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG),对136只母兔进行超数排卵处理试验。结果显示,国产的高活性FSH对母兔有良好的超排作用。试验母兔中有85%表现超排效应,平均每只母兔可回收到31.1个卵,几乎相当于对照组(自然发情组,8.5个/只)的4倍。母兔对超排的反应表现出明显的季节性,春季时的处理效果极显著地优于秋季。其中FSH组在春、秋季的回收卵数分别为35.0个/只与11.9个/只,差异十分显著(P<0.001);PMSG组也表现类似效果。另外,注射HCG后不同回收时间对卵回收数的影响也很大。在注射了HCG13.5h后卵母细胞的回收数非常显著地比13.5h前高,两者几乎相差1倍(36.0:18.3)。同时,母兔左、右侧卵巢排卵数也表现出明显差异,不论是自然发情或人工超排,母兔右侧卵巢的排卵数均显著地高于左侧卵巢。
The effects of FSH (made in China or Sigma), PMSG and HCG on superovulation of 136 female rabbits during 1991 ̄1993 are reported. Results indicate that the high active FSH (made in China) has a very good superovulation effect on rabbits. Among the treated animals, 85 % responded to the treatment and an average of 31. 1 ova per rabbit were collected, which was nearly 4 times than that of the natural oestrous rabbits (8. 5).The superovulation response of rabbits is better in Spring than in Autumn. The average number of ova collected from FSH-treated rabbits in Spring (35. 0) was significantly greater than that in Autumn (11. 9, P<0. 001), and so was the case in PMSG-administrated rabbits. In addition, the ova collection time had a clear effect on the number of ova harvested. The number of ova collected more than 13. 5 h after HCG injection was nearly double of ova collected with in 13. 5 h (36. 0 vs 18. 3). Significantly more ova were ovulated from the right ovary than from the left one during both natural oestrous and superovulation.
关键词
兔
促性腺激素
超数排卵
排卵数
rabbits
gonadotrophin
superovulation
ovulation number
seasonal effect
ova collection time