摘要
应用样品一个稀释度间接ＥＬＩＳＡ法对产蛋母鸡及其小鸡进行传染性法氏囊病病毒（ＩＢＤＶ）抗体效价测定，同时用病毒中和试验（ＶＮ）作比较检测。结果表明，该ＥＬＩＳＡ法比ＶＮ法敏感、精确、简便，所有样品只测定一个稀释度的Ｐ／Ｎ值，再根据线性回归方程（ｒ１=０．８８９８，Ｐ≤０．０５）计算抗体效价，达到省时省力、节约材料、减少试验误差和一次可进行大批样品检测的目的。２种方法相关性较高（ｒ３=０．８４９２，Ｐ＜０．０１），小鸡母源抗体衰减与日龄呈线性关系（ｒ２=－０．８７５８，Ｐ＜０．０１），ＩＢＤＶ母源抗体半衰期为６．３ｄ。
The hens and their respective progenies were tested for antibody titers against infectious bursal disease virus(IBDV) by the indirect ELISA in a single serum dilution and compared with virus neutralization VN)test. The indirect ELISA was more accurately and rapidly in predicting antibody titers than the VN test (P≤0. 05), but they had a high correlation coefficient of 0.849 2(P<0. 01 ).The half-life of the chick's maternal antibody was 6.3 days,had linear correlation with the days (P<0. 01).
关键词
ELISA法
抗体效价
母源抗体
鸡病
IBD
infectious bursal disease virus
indirect ELISA in a single serum dilution
virus neutralization test
antibody titer
maternal antibody
half-life
