Se、Cu与AFB_1相互作用对细胞遗传物质影响的实验研究

THE EFFECT OF THE INTEKACTION BETWEEN TRACEELEMENTS SELENIUM AND COPPER WITH AFLATOXINB_1 ON THE GENETIC MATERIAL

以正常人外周血淋巴细胞的ＳＣＥ率作为细胞遗传学指标，研究了Ｓｅ、Ｃｕ与ＡＦＢ＿１相互作用对细胞遗传物质的影响。实验结果表明Ｃｕ具有明显的致变作用，在１０￣（－１０）～１０￣（－４）ｍｏｌ浓度范围与ＡＦＢ＿１无明显协同或抬抗作用；而Ｓｅ则具有较好的桔抗Ｃｕ及ＡＦＢ＿１的致变作用，但在１０￣（－２）ｍｏｌ浓度以上具有一定细胞毒性作用。提示Ｓｅ可作为防致突变剂，但在应用时应考虑其双向作用。

Normal human lymphocyte sister-chromatic exchange(SCE)as an indicator ofgenetic material injured by mutagenic agent,was used to investigate the effect ofthe interactionbetween trace elements selenium and copper with aflatoxin B1 on the genetic material.The ex-perimental result showed that there was no significant synergic or antagonic effect between tracecopper and aflatoxin B1 when CuSO4 concentration was within 10-10to 10-4 mol.The SCE valueinduced by AFB1 was depressed by CuSO4 in the concentration of 10-3 mol.Whether copperhas a biphasic effect or the two mutagenic agents have a comprehensive cytotoxic effect on thegenetic material is worth further studying.se concentration of 10-5to 10-4 mol inhibited the mu-tation induced by copper or AFB1.This study also showed that trace element Se was cytotoxicwhen its concentration was higher than 10-2mol.Such result could be explained by the fact thattrace element Se also had a biphasic effect on the genetic material.The research suggests that thequantity of Se must be well considered when it is used as an agent for cancer prevention.