亚硝酸钠与AFB_1相互作用对遗传物质影响的实验研究

RESEARCH ON THE EFFECT OF THE INTER-ACTION BETWEEN SODIUM SELENITE AND AFLATOXIN B1 ON CYTOGENETIC MATERIAL

以正常人外周血淋巴细胞的ＳＣＥ率作为细胞遗传学指标，研究了Ｎａ＿２ＳｅＯ＿３与ＡＦＢ＿１相互作用对细胞遗传物质的影响。结果表明，一定浓度的Ｎａ＿２ＳｅＯ＿３（１０￣（－５）ｍｏｌ）对ＡＦＢ＿１所诱发的ＳＣＥ有明显的抑制作用，但当浓度达到１０￣（－２）ｍｏｌ时，细胞增殖受到抑制，到１０￣（－１）ｍｏｌ时细胞出现毒性现象。提示Ｎａ＿２ＳｅＯ＿３具有抑变和细胞毒性双相作用。所以在Ｎａ＿２ＳｅＯ＿３与ＡＦＢ＿１相互作用的ＳＣＥ实验中应避免Ｎａ＿２ＳｅＯ＿３的浓度过高而损伤培养的细胞。

Using the cytogenetic method,the effect of the interaction between sodiumselenite:and aflatoxin B1 on cytogenetic material was studied with the peripheral bloodlymphocyte sister chromatic exchanges(SCE)as an index of the cytogenetic material damagedby mtitagenesis in vitro. The results showed that sodium selenite in certain concentration(10-5 mol)inhibited SCE rate induced by AFB1.When the concentration of sodium selenite was10-2 mol,the proliferation of the cells wns inhibited. When the concentration of sodium selenitewas 10-1 mol cytotoxicity appeared. The results suggested that sodium selenite had a dual effect on antimutagenessis and cytotoxicity. So in SCE test too high concentration of sodium seleniteshould be avoided in order to prevent cytonecrosis.