改良ELISA测定结核抗体的意义

THE SIGNIFICANCE OF DETECTING MYCO-BACTERIUM TUBERCULOSIS ANTIBODYBY IMPROVED ELISA

报道３，３’，５，５’－四甲基联苯胺（ＭＢ）用于ＥＬＩＳＡ检测血清中结核抗体的水平。结果：其敏感性为８５．２％，特异性为９８．５％，且底物于反应终止后呈鲜蓝色，久置不会加深，阴、阳性对比十分明显，既利于目测，又可用酶标仪准确测定。提示此底物可明显改善实验条件，值得推广应用。

This study reports the use of 3,3’,5,5’-tetramethylbenzidine(TMB)as asubstrate of ELISA for detecting microbacterium tuberculosis antibody.The results show that ithas a sensitivity of 85.2% and a specificity of 98.5%. It is more stable and maintains the brightblue color 28 hours or more after the reaction has stopped. Positive or negative results can bedistinguished easily by the naked eyes or a spectrophotometer,suggesting that the substrate canimprove the conditions of the laboratory management.Because of the unique characteristic ofthe improved ELISA described above, it is certainly worthwhile to introduce it for laboratoryinvestigation.