丁型肝炎病毒感染对乙型肝炎病毒复制的影响研究

A STUDY OF THE EFFECT OF HDV INFEC-TION ON HBV REPLICATION

用ＥＬＩＳＡ测定１８１例各型ＨＢＶ感染者血请中ＨＤＶ标志物，结果ＨＤＶＭ阳性５７例，阳性率为３１．４９％。各型肝炎间ＨＤＶ感染卒的差异有显著性（Ｐ＜０．０５），依次为ＳＨ＞ＬＣ＞ＣＡＨ＞ＣＰＨ＞ＡＨ＞ＡＳＣ。用异羟基洋地黄毒试元标记的ＨＢＶＤＮＡ探针测定其中５７例ＨＢＶ／ＨＤＶ及单纯ＨＢＶ感染病人血清中ＨＢｖＤＮＡ及其含量，结果ＨＢＶＤＮＡ的检出率在ＨＢＶ／ＨＤＶ组（６７．９２％）明显低于单纯ＨＢＶ感染组（８４．６２％）（Ｐ：０．０１５２），且前组中ＨＢＶＤＮＡ含量也明显低于后组（Ｐ＜０．０５）。以上结果提示：ＨＤＶ感染多见于严重的及慢性肝脏疾病；在ＨＢＶ／ＨＤＶ感染的急性期，ＨＢＶ复制受到抑制；而在重症型、慢性期和肝硬化时则未见这种抑制现象，说明在ＨＢＶ／ＨＤＶ感染的不同阶段可表现出不同的病毒复制现象。

Serum hepatitis D virus marker were tested by ELISA method in 181 HBV in-fected patients.HDV infection rate was 31.4%,Serum HBV-DNA was determined using spothybridization technique with Digoxin-labeled HBV-DNA probe in 157 patients with HBV in-fection and HBV/HDV infection. The HBV-DNA positive rate was 78.9%. SerumHBV-DNA was detected in 36 of 53(67.92%) patients with HBV/HDV infection and in 88 of104(84.62 %)patients with simple HBV infection. There was a signicant difference betweenthese two groups(P<0.05).HBV-DNA was present in patients with HBV/HDV infection atlow levels(trace to 2+)and in patients with simple HBV infection at high levels(2+ to 4+).TheHDV infection rates in several hepatitis types were of the following order:SH>LC>CAH>CPH>AH>ASC(P=0.018).Our study shows that HDV infection is higherand is mainly found in those patients with severe and chronic liver diseases.The replication ofHBV is suppressed during the acute phase of HBV/HDV infection,but not in HBV/HDVinfectied patients with SH, CH or cirrhosis.These findings indicate that different stages ofHBV / HDV infection may show different virus replication.