摘要
在６、７—Ｖ培养基中适合中国黄连愈伤组织生长的蔗糖浓度为３％，综合考虑愈伤组织生长较慢兼顾小檗碱含量，较为适合的蔗糖浓度为４％。在培养基中补加０．８ｇ／Ｌ硝酸铵对愈伤组织鲜重、干重和小檗碱含量均有不同程度的提高。
The proper sucrose concentration for callus growth of Coptis chinensis was 3%in 6.7─V media,but the more suitable concentration was 4%concerning with slower callus growthin the concentration and raising berberine content comprehensively.When additional NH4NO3in different concentration was added to the media,it was showed that fresh and dry weightof callus and berberine content were raised in varied degree in the concentration of 0.8g /L.
出处
《贵州农业科学》
CAS
1994年第3期15-18,共4页
Guizhou Agricultural Sciences
基金
贵州省科委资助
关键词
黄连
蔗糖
硝酸铵
愈伤组织
Coptis chinensis
sucrose
ammonium nitrate
callus
berberine
