番茄组培苗过氧化物酶同工酶分析

ANALYSIS OF ISOZYMES OF PEROXIDES OF TISSUE-CULTURED TOMATO PLANTS

本实验对番茄组织培养过程中分化产生的愈伤组织、根、芽、茎、叶等进行了过氧化物酶同工酶分析，并以生长１５－２０天的正常番茄苗的根、茎、叶材料作为对照。结果表明，组培植物的过氧化物酶同工酶酶谱与对照有较明显变化，组培材料酶谱带染色较深，其中根中Ａ—３—α，Ｂ—１—α带，愈伤组织的Ａ—３—α带显示最深，组培叶显示了１７条带，而对照叶仅显示了５条带；茎显示１７条带，而对照显示６条带，尤其是组培材料均显示出Ｂ—３带，而对照材料没有此条带．上述结果对探讨组织培养过程中分化的生理基础具有一定意义．

Isozymes of peroxides from callus, roots, buds, stems, leaves of tissue-cultured tomatohave been analysed using the roots, stems and leaves of 15-day-old normal tomato plants as controls. Following results wers obtained: Enzyme pattems of isozyme of peroxides from tissue-cultured materials is great different from contrals'. Enzyme pattrns of tissue-cultured materials is dancer than controls'. B-1-2 bands of roots and A-3-a bands of callus is darkest. Leaves of tissue-culted tomatos indicate sex,enteen bands and controls' only six bands. Especially, all tissue-cultured materials have the B - 3 band, but control material haven it. Preceding conclusions is hepful to the research of physicalmechanism of differentiation of tissue cultrue.