摘要
本实验对番茄组织培养过程中分化产生的愈伤组织、根、芽、茎、叶等进行了过氧化物酶同工酶分析,并以生长15-20天的正常番茄苗的根、茎、叶材料作为对照。结果表明,组培植物的过氧化物酶同工酶酶谱与对照有较明显变化,组培材料酶谱带染色较深,其中根中A—3—α,B—1—α带,愈伤组织的A—3—α带显示最深,组培叶显示了17条带,而对照叶仅显示了5条带;茎显示17条带,而对照显示6条带,尤其是组培材料均显示出B—3带,而对照材料没有此条带.上述结果对探讨组织培养过程中分化的生理基础具有一定意义.
Isozymes of peroxides from callus, roots, buds, stems, leaves of tissue-cultured tomatohave been analysed using the roots, stems and leaves of 15-day-old normal tomato plants as controls. Following results wers obtained: Enzyme pattems of isozyme of peroxides from tissue-cultured materials is great different from contrals'. Enzyme pattrns of tissue-cultured materials is dancer than controls'. B-1-2 bands of roots and A-3-a bands of callus is darkest. Leaves of tissue-culted tomatos indicate sex,enteen bands and controls' only six bands. Especially, all tissue-cultured materials have the B - 3 band, but control material haven it. Preceding conclusions is hepful to the research of physicalmechanism of differentiation of tissue cultrue.
出处
《哈尔滨师范大学自然科学学报》
1994年第2期98-101,共4页
Natural Science Journal of Harbin Normal University
基金
蔡火石生物科学发展基金