利用Talphlb综合体进行Tal基因转移的研究

STUDY OF Tal GENE TRANSFERED BY Tal Phlb COMPLEX

本文利用人工合成的Ｔａｌｐｌｉｌｂ综合体，进行了核不育硬粒小麦的选育工作，得到“柯－３”号株系，其Ｆ１、Ｂ１Ｆ２、Ｂ２Ｆ３代的育性分离比（不育株／可育林）始终接近１：１，与太谷核不育小麦的育性分离比相似，推测“柯－３”号株系为具４０条染色体的易位系。对回交后代育性分离情况进行分析，发现总体上后代不育林比例下降，但株系调查表明，有的株系（如柯－３）自Ｂ１Ｆ２代起不育株／可育株的化例始终接近１：１，推测在Ｆ１代即发生了染色体易位或同祖染色体的交换，这是利用Ｔａｌｐｈｌｂ综合体的作用所致。

In this paper,male sterile durum wheat were selected by Tal phlb comlplex, and obtained a strain line of Ke-3. It's fertile segregation ratio as same as male sterile Taiguline (sterile lines to fertile lines) approaches one to one in F1, B1F2 and B2F3, respechveley. We though that Ke-3 w 4D translocation line. Backeross offspring fertile segregation were analysed that the ratio of offspring sterile line was decreased. Some line such as Ke-3 ration (sterile lines to fertile lines) approaches one to one as a whole. It is support the F1 generation have 4Dchromosome tianslocation or exchange of homologous chromosomes These is due to phlb complex.