用HIL系列单抗筛选和研究癌转移相关基因

Screening and Studying Metastasis Associated Gene with HIL Monoclonal Antibodies

用ＨＩＬ系列单抗筛选和研究癌转移相关基因病理学教研室齐滕平，李仁，毕彦忠，班翔，王吾如肿瘤研究所徐明旭，史历，谭玉英本实验室近１０年来对癌细胞转移机制进行了研究，曾从低转移性人肺腺癌细胞系ＡＧＺＹ－８３ａ中筛选和建立了高转移性细胞亚系Ａｎｉｐ９７３，...

Monoclonal antibodies were used to identify a positive staining clone from a λZAP Ⅱ expression library,which was derived from a highly metastatic human lung adenocarcinoma cell line。The cDNA was isolated and its nucleotide sequence was determined,DNA and RNA dot blot hybridization showed that the level of mRNA expression was higher in the highly metastatic cell line than that in the poorly metastatic cell line.The cDNA contained an open reading frame of 106 amino acids and revealed 85% homology with a human propionylCoA carboxylase α-chain