胎肌条件培养液对白血病细胞的体外效应

In vitro effects of fetal muscle condition medium on leukemic cells

以髓系(HL-60)与淋系(Molt-4)细胞为观察对象,用细胞计数、细胞集落培养、台酚蓝拒染及溴化二甲噻唑二苯四唑试验为观察手段,研究胎肌条件培养液(FMCM)对白血病细胞的影响.实验标本分为4组:①对照组.②20%FMCM处理组.③阿糖胞苷(Ara-C,1×10^(-7)M/L)处理组.④混合处理组(经FMCM与Ara-C二者的处理).发现:①FMCM对两个细胞株均无明显的直接作用.②当HL-60细胞经FMCM预处理或在用Ara-C处理同时加入FMCM,Ara-C对其抑制作用可为FMCM加强,已受Ara-C抑制的HL-60细胞则无此现象.③Ara-C对Molt-4的抑制作用不受FMCM的影响.由此而初步认为:①FMCM对白血病细胞似无明显直接作用.②某些(非全部)白血病细胞对Ara-C的敏感性可为FMCM加强.③在己受抑制的细胞中不呈现这种致敏现象.①提示FMCM有可能用于白血病临床治疗.

The effects of fetal muscle condition medium (FMCM) on the biologic performance of HL-60 (myeloid line) and Molt-A (lymphoid line)were evaluated with criteria of cell count, colony formationculture, trypan blue exclusion and MTT assay. Samples were devided into 4 groups: (1)Control. (2)20%FMCM treated group.(3) Arabinoside C (Ara-C,1×10-7M/L)treated gruop.(4)Combine-treated group (treated by both FMCM and Ara-C). It was found that: (1)There was no obvious direct effect of FMCMon both cell lines.(2) The inhibition effect of Ara-C on HL-60 cells could be enhanced by FMCM whenthose cells were pretreated with FMCM or treated simultaneously with FMCM. (3)Inhibition effect ofAra-C on Molt-4 cells could not be altered by FMCM. It is evident from our observation that (1) Itseems there was no obvious direct effect of FMCM on leukemic cells .(2)Sensitivity to Ara-C of certaintypes of leukemic cells could be enhanced by FMCM.(3)Such sensitization did not exist in the cells already inhibited by cytotoxic drugs. There could be a potential of FMCM for leukemic therapy.