^（99m）Tc直接标记IgG及其稳定性研究

The direct labelling of IgG with￣^(99m)Tc and its stability studies

采用二硫苏糖醇还原ＩｇＧ和合亚锡的药盒还原高锝酸盐法将９９ｍＴｃ直接标记ＩｇＧ。其标记率＞９５％，标记后无需纯化。使用多种弱交换配体（亚锡ＰＹＰ、ＭＤＰ、ＧＨ、Ｐｈｙｔａｔｅ和ＥＨＩＤＡ药盒）获得了高的标记率，但此法不宜用ＤＴＰＡ药盒．体外研究和显像表明９９ｍＴｃ－ＩｇＧ的稳定性良好，可用于局部实验性脓肿的诊断。

he immunoglobulin G(IgG) was directly labelled with 99mTc by prereduction of IgG with dithiothreitol and redution of pertechnetate wiht a stannous kit. The radiochemical purity was over 95% .Hence no purification was needed,High labelling dfficiency could be obtained with a variety of weak exchange ligands, such as PYP, MDP, GH, Phytate and EHIDA kits ,But DTPA was not effective substance for this procedure. iN vitro studies and the animal imaging showed that 99mTc -IgG was stable useful in diagnosis of focal experimental abscess.