铁（Ⅱ）－1，10，邻菲啰啉－碘化物三元配合物萃取－分光光度法测定微量铁

Microsynthesis of lron by Extraction-Spectrophotometry of Fe(Ⅱ)-1,10-Phenanthroline-lodide Ternary Complex

铁（Ⅱ）与１，１０－邻非啉及碘化物形成离子缔合型三元配合物，用三氯甲烷作萃取剂，分光光度法测定有机相中的微量铁。确定分析条件：最大吸收波长为５１５ｎｍ；缓冲溶液采用乙酸－乙酸按溶液，其ｐＨ值为５．８，用量为５．０ｍｌ；０．１５％邻菲啉用量为５．０ｍｌ，２０％碘化钾用量为０．８ｍｌ。测定人发中的微量铁，相对标准偏差为０．６７％、．６６％，加标回收率为９７．３％～１００．８％。

Micro Fe(Ⅱ)in organic phase is combined with iodide and 1, 10-phenanthroline, and forms a ternary complex of ionic association, using chloroform as extracting agent,then is determined by spectrophotometry. Defined analytic conditions are:maximum absorption at 515 nm; using acetic acid-ammonium acetae solution as buffer,pH=5.8,amount of it is 5.0 ml a time;amount of phenanthroline of 0.15 %is 5.0 ml a time,amount of potassium iodide of 20 %is 8.0 ml a time. Determination of micro iron in hair has relative standard deviation of 0.67% ,1.66%, recovery of 97.3%～100.8%.