适用于我国小麦醇溶蛋白分析的APAGE方法

A Practical APAGE Method of Wheat Gliadin Analysis in China

本文依据国际种子检验协会（ＩＳＩＡ）颁布的小麦、大麦品种鉴定的酸性聚丙烯酰胺凝胶电泳（ＡＰＡＧＥ）方法，利用国产仪器、药品对其做了适合性检验，并就凝胶浓度、交联度及强度做了调整。对千余份普通小麦的ＡＰＡＧＥ分析表明，改良后的方法效果稳定，具有谱带分辨率高、重现性好、胶强度适宜、易剥离、制成干板后谱带不扩散及操作简便、快速等特点。认为是一套适合我国试验器材和药剂等实际条件的麦醇溶蛋白分离方法，可以做为品质研究、品种鉴定的可靠分析手段。

with home-made electrophoretic instruments and reagents, the APAGE method of wheat gliadin recommended by ISTA was improved, in gel concentration and strength. The improved APAGE method showed a stable separation in gliadin protein according to the electrophoretograms of more than one thousand common wheat varieties. In addition, this improved method has also been proved to have characters of high resolving power, appropriate gel strength, simple and rapid to use and bands in dry slab without spreading. Thus, it was regarded as a practical technique for separating wheat gliadin protein in China.