BR316降解2．4—D的基因克隆

CLONING OF HERBICIDE 2.4-D DEGRADATION GENE OF BR316

本文在对ＢＲ３１６质粒遗传特性研究的基础上，对质粒进行了克隆，经α互补特性观察，重组子ＤＮＡ的酶切分析和重组子在ＤＰ１培养基上的生长情况观测，结果表明重组子具有目的基因降解２．４—Ｄ的特性，并能充分表达和较稳定传代。

A bacterium BR316 can degrade herbicide 2.4-D, degrading-characteristic is encodened by plasmid,size of 4. 6 Kb. Preliminary cloning has been established on basis of plasmid studied by ligating vector Puc18 and plasmid DNA. a complement and analysis of recombinant incised by enzyme have showed that recombinant from Pucls and target plasmid DNA has been obtained.