摘要
研究结果表明,N_6、MS等14种培养基对曲茎石斛、铁皮石斛的种胚成苗率、种胚苗生长有显著作用,其中以N_6为佳。在1/2N_6培养基内添加香蕉汁(150mg/L),石斛种胚苗生长最好;若添加2mg/L或1mg/LNAA,可提高石斛种胚苗分蘖率6~7.14倍。选曲茎石斛或铁皮石斛的茎尖、茎段、充实幼叶及其种胚、鳞叶期原球茎、种胚苗、幼根为外殖体,可培育出大批组培苗。栽培试验结果表明,石斛组培苗在特殊的环境条件下,5月上中旬栽培。用苔藓或腐乳质土覆盖幼根,成活率达95%以上,且生长发育良好。
The research results showed that the embrvos of Dendrobium flexicaule Z.H.Tsi,S.D.San et L.G.Xu and D.candidum Walex Lind.could be cultured on N_6,MS andother medium. Among them N_6 was the best fellowed by SH and MS.The embryo seedlingsgrew best on 1/2 N_6 with 150mg/L of banana(Musa nana Linn.) juice adding to.When1mg/L or 2mg/L of NAA was odded, the tillering rate was increased by 6~7.14 times.Many parts of the D.flexicaule and D.candidum such as tip, part of the stem full shapeyoung leaf,embryo,embryo seedling and part of the young root could be cultured on N_6 orMS medium, and lots of tissue cultured seedlings could be bred.Planting results showedthat tissue cultured seedlings must beplanted under such special conditions as in the mid-dle of May,and covered by bryophyte or wet humus soil.The survival rate was over 95%,and the seedlings grew well.
出处
《河南农业大学学报》
CAS
CSCD
1994年第2期128-133,共6页
Journal of Henan Agricultural University
关键词
石斛
组织培养
放栽
Dendlobium
tissue cultare
planting technique