污染水果的霉菌提取物诱发微核实验

Provocative Micronucleus Test on the Extracts of Mycetes from Polluted Fruits

应用小鼠骨髓嗜多染红细胞微核试验，分别对从市售污染水果梨和桔子中分离出的优势真菌互隔交链孢霉和指状青霉提取物进行诱变实验。结果：菌株Ａ６和菌株Ｐ３提取物分别在达到２００ｍｇ／ｋｇ、１６０ｍｇ／ｋｇ时，其微核出现率分别为１６．８±０．３９‰、１８．２±０．３８‰，与溶剂对照组（ＤＭＳＯ）３．６０±０．４６‰相比有显著性差异（Ｐ＜０．０１），并呈明显的剂效关系。说明互隔交链孢霉和指状青霉两种提取物含有使染色体损伤的物质。

Mutagenic experiment on the extracts of dominant Alternaria alternata and Penicillium digitatum from the polluted fruits(pears and oranges)sold in the market was carried out with the micronucleus test on the polychromatic erythrocytes in the marrow of the mouse.The results showed that when the extracts of strain A6 and P3 reached 200 mg/kg and 160 mg/kg respectively,the micronucleus occurrence rates were l6.8±0. 39%and 18.2±0.38%,which were significantly different from the rate 3.60±0.46%of the solvent control group(P<0.01),and had a dosage-response relationship.The above findings show that the extracts of Alternaria alternata and Penicillium digitatum contain the materials which can damage the chromosomes.