小鼠Lewis肺癌热耐受动力学研究

STUDY ON KINETICS OF THEKMOTOLERANCE IN MOUSE BEARING LEWIS LUNG CARCINOMA

我们用荷Ｌｅｗｉｓ肺癌的ＮＩＨ小鼠，以平均直径倍增时间为终点，进行热耐受动力学研究。将带瘤腿浸入４３±０．１℃的水浴中进行局部加温。小鼠以腹腔注射６０ｍｇ／ｋｇ戊巴比妥钠麻醉。在４３℃加温２５ｍｉｎ后，间隔０，８，１６，２４，４８，７２，９６，１２０，１４４，１６８，１９２，２１６和２４０ｈ再给第２次加温。结果表明，４３℃加温２５ｍｉｎ后，肿瘤迅速产生热耐受性，在２４ｈ达到最大值，然后逐渐减弱，而在９６ｈ后减弱很慢。热耐受性的存在，减弱第二次加温的疗效。

The kinetics of thermotolerance was studied on NIH mouse bearing lewislung carcinoma by mean diameter doubling time(MDDT)as the end point.Local hyperther-mia was given by immersing the tumor-bearing foot into the water bath kept at 43±0.1C.NIH mice were anesthetized by intraperitoneal injection of pentobarbitone sodium at 60 mg/kg body weight.After 43℃ 25 min heating,at intervals of0,8,16,24,48,72,96,120,144,168,192,216 and 240 h,the second heating was done to NIH mouse bearing lewis lung carci-noma. The results showed that after 43℃ 25 min heating,the tumor rapidly developed ther-motolerance which reached the maximum at 24 h and then gradually weakened,and veryslowly weakened after 96 h.The existence of thermotolerance weakened the therapeutic effi-ciency of second heating.