高脂血清对人内皮细胞前列环素、脂质过氧化物的影响及维生素E的保护作用

EFFECT OF HYPERLIPIDEMIC SERUM ON LIPID PEROXIDATION,SYNTHESIS OF PROSTACYCLIN BY CULTURED HUMAN ENDOTHELIAL CELLS AND PROTECTIVE EFFECT OF VITAMIN E

实验比较了高脂兔血清（ＨＲＳ）和正常兔血清（ＮＲＳ）对培养的人脐静脉内皮细胞（ＥＣ）脂质过氧化物（ＬＰＯ）的形成和ＥＣ利用外源性花生四烯酸（ＡＡ）合成前列环素（ＰＧＩ２）能力的影响；同时观察了抗氧化剂ＶｉｔＥ对ＥＣ的保护作用。结果表明，ＨＲＳ对ＥＣ合成ＰＧＩ２的影响有一时间和浓度相关的反应过程。用ＨＲＳ孵育培养呈融合状的ＥＣ，在４８ｈ前刺激ＰＧＩ２的产生，４８ｈ达最高值，然后急骤下降；而ＬＰＯ含量随着孵育时间的延长呈直线上升（ｒ＝０．９８７）。随着ＨＲＳ浓度的增加，ＬＰＯ含量逐渐升高，ＥＣ合成ＰＧＩ２能力呈下降趋势。ＶｉｔＥ可阻止ＨＲＳ引起的ＥＣ中ＬＰＯ含量的增加和ＰＧＩ２合成能力的下降。

The purpose of this experiment was to compare the effects of hyperlipidemicrabbits serum (HRS)and normal sera(NRS)on lipid peroxide (LPO)content and prostacy-clin(PGI2)synthersis by cultured human vascular endothelial cells (EC)which utilizing ex-ogenous arachidonic acid,and to observe the protective effects of antioxident Vitamin E.Theexperiment showed that HRS induced a time-and does-dependent alteration in the productionof PGI2 and the levels of LPO in EC.EC incubated with HRS increased PGI2 generation be-fore 48 h,and then decreased.At the 48th hour had a maximum leve1. The LPO levels contin-uously increased with the inctrase of time.There was a positive correlation between the con-centration of HRS and the levels of LPO and an inverse correlation with PGI2 synthesis. Vita-min E could inhibit the increase of LPOand decrease of PGI2.