用聚合酶链反应法检测172例孕妇尿中巨细胞病毒－DNA

DETECTED HCMV-DNA IN URINE OF 172 CASES PREGNANT WOMEN

本文对１７２例孕妇的尿用聚合酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎＰＣＲ）进行巨细胞病毒－ＤＮＡ检测（ｈｕｍａｎｃｙｔｏｍｅｇａｌｏｖｉｒｕｓＨＣＭＶ－ＤＮＡ），结果显示非正常孕妇（以前有自然流产、死胎、畸胎、不育症等）１２１人尿中ＨＣＭＶ－ＤＮＡ阳性者４３例，阳性率为３５．５％，而５１例正常孕妇中阳性者仅２例，阳性率为３．９％，表明活动性人巨细胞病毒感染是非正常孕妇的重要原因之一。２０例孕妇尿中ＮＣＭＶ－ＤＮＡ阳性者用干扰素、转移因子等治疗后，１４例转阴。用ＰＣＲ法检测活动性人巨细胞病毒感染是一个比较准确、敏感、快速的方法，并是保证优生优育的重要手段之一。

With PCR to detect HCMV-DNA in urine of 172 cases pregnant women.Theresults showed that 1 2 1 cases abnormal pregnant women(They and born formerly mal-fomed fotus,sterility,spontaneous ahortion or stillbirth etc)were 43 cases positive of HCMV-DNA in urine,the positive rate was 35.5%.But 51 cases normal pregnant women had only 2cases positive of HCMV-DNA in urine,the positive rete was 3.9%. The results shou that ac-tive infection of HCMV are one of major reason of abnormal pregnant women. Twenty casesof abnormal pregnant women which HCMV-DNA in urine was positive were treated with in-terferon or transfer facter etc,14 cases of them were change into negative. With PCR to de-tect active infection of HCMV were a sensitive,accurate,quick method,and PCR is an impor-tance measures to assure ellgenic and excellent birth.