补体抑制免疫复合物沉淀作用的实验研究

EXPERIMENTAL STUDY ON INHIBITION OF IMMUNE PKECIPITATION BY COMPLEMENT

本文以辣根过氧化物酶（ＰＯ）作抗原，与相应抗体（抗－ＰＯ）混合，加入血清，对血清补体抑制免疫复合物沉淀作用（ＩＩＰＣ）进行了研究。结果，在抗原抗体最适比例时形成免疫复合物最多；原血清及１：２稀释血清ＩＩＰＣ最强，ＯＤ值为０．５７和０．５４，血清的进一步稀释对ＩＩＰＣ有一定影响；孵育时间以３０ｍｉｎ最宜，ＯＤ值为０．５６；离心时间在２５～４５ｍｉｎ时ＯＤ值最大为０．５０～０．５６；在最适条件下，正常人混合血清ＯＤ值为０．４９；５６℃３０ｍｉｎ灭活血清为０．１４；５０℃１５ｍｉｎ处理血清为０．３８；ＥＤＴＡ处理血清为０．０９；ＥＧＴＡ处理血清为０．２１；对２０例健康人血清ＩＩＰＣ测定结果为０．３９～０．６２，平均０．４８±０．０７。表明正常血清补体对免疫复合物的沉淀有明显抑制作用。各种因素处理血清对ＩＩＰＣ均有一定影响，而以５６℃３０ｍｉｎ和ＥＤＴＡ处理血清最明显。文中还对该法的影响因素进行了讨论。

In this paper,a method for measuring inhibition of immune precipitation bycomplement (IIPC) using peroxidase(PO)as an antigen was studies. The results showed thatimmune complex of PO and anti-PO rabbit IgG at equivalence could form a great quantity ofprecipitation. OD values were 0. 57 and 0. 54 in non and diluted serum (1 : 2),0. 56 in 30 minincubation time,and 0.50～0.56 in 25～45 min centrifugation time. OD value of normal indi-viduals mixture serum ,the serum of heating to 56C for 30 min ,50℃ 15min ,and treated by-ethylenediamine tetracetate (EDTA) ,ethyleneglycol tetracetate(EGTA ) were 0.49,0.14,0, 38,0. 09,0. 21 respectively. IIPC of 20 normal persons was 0. 48 + 0. 07(0. 39～0.62).These results indicate that normal serum markedly inhibites precipitation of immune complexand many agents may block IIPC of serum ,especially 56℃ for 30 min and EDTA,The vari-ous factors of inhibiting IIPC are discussed in the paper.