肝癌旁组织的流式细胞DNA含量分析

Flow Cytometric Study of Cellular DNA Content of Peri-Cancer Tissues in Hepatocellular Carcinoma

本文用流式细胞术对４１例原发性肝癌患者癌灶和癌旁２、４、６、１０ｃｍ肝组织的１０８个样品进行了ＤＮＡ含量及其分布测定。结果表明，肝癌癌灶的ＦＣＭ阳性检出率为７５．６％，癌旁组织为５８．２％。癌旁不同距离的肝组织ＦＣＭ阳性率之间无明显统计学差异。在ＦＣＭ阳性检例中，异倍体肿瘤细胞向周围组织侵袭能力最强，近二倍体肿瘤细胞最弱，其余类型介于二者之间。癌灶细胞ＤＮＡ指数大部分集中在３～４倍体附近，癌旁组织则集中在二倍体附近。癌旁各点的ＤＮＡ指数和高二倍体细胞百分率均与离癌灶的距离呈明显的负相关（ｒ分别为０．８１和０．９２）。

DNA content was measured on 108 samples of peri-cancer tissues which were taken at 2, 4, 6 and 10cm sites departing from tumors, and the cancer nests in 41 patients suffering from hepatocellular carcinoma. The results showed that the positive rate of FCM was 75. 6% in the cancer nests, while the rate was 58. 2% in the pert-cancer tissues. The rate had no significant difference between various sites of the pert-cancer tissues. The dissminated capability of AN (qneuploid),cells was strongest in the. cases if the positive rate of FCM in abnormal ploid type, while this capability of ND (neardiploid) cells was lightest. DI (DNA index) value of the cancer nest distributed about triploid-tetraploid, while the value of the adjacent tissue dispensed about diploid. DI value and >2C% (high diploid cells percentage) in different sites of peri-cancer tissues have significant minus interrelation (r=0. 81 and r=0. 92)with the distance of the cancer nest.