外源DNA完全酶解成分在水稻浸胚法导入中的诱变作用

THE MUTATION-INDUCING FUNCTION OF COMPLETELY DIGESTED DNA DURING THE EMBRYO-SOAKING INTRODUCING OF EXOGENOUS DNA INTO RICE

外源ＤＮＡ以ＤＮＡ酶（ＤＮａｓｅⅠ）完全酶解后，浸泡处理灿型中优早２号糙米，研究了外源ＤＮＡ酶解成分在水稻浸胚法导入中的诱变作用。处理当代即表现了一定程度的变异，表明浸胚法外源ＤＮＡ导入过程中确存在着完全降解成分的诱变作用。

The mutation-inducing function of the elements of the completely digested DNA treated with nuclease(DNase 1) was studied by means of soaking the rice grains of Zhongyouzao 2 with the digested DNA solution.Mutations found at some extant in the soaked generation.It showed that some mutations were induced by the elements of complete-ly digested DNA during embryo-soaking introducing of foreign DNA into rice.