重症肝炎病人外周血单个核细胞IL－2的产生及其受体表达

IL-2 LEVEL AND EXPRESSION OF IL-2R ON PERIPHERAL BLOOD MONONUCLEAR CELLS IN THE PATIEMTS WITH SEVERE HEPATITIS

观察外周血单个核细胞经植物血凝素刺激２４ｈ后ＩＬ－２受体表达情况，结果如下：２９例重症肝炎病人ＩＬ－２受体为３８．０８±１４．０１％，１３例慢活肝病人为３５．５０±１３．００％，２０例正常人为６０．５５±１７．７９％。重症肝炎、慢活肝病人ＩＬ－２受体表达功能明显受损，且与正常人有较大重叠，与病情轻重无密切关系。ＩＬ－２受体表达下降由多种因素所致。

nterleukin- 2 receptor(IL- 2R)in peripheral blood mononuclear cells(PBMC)in 29 casesof severe hepatitis and 13 cases of chronic active hepatitis was determined by indirect im-munofluorescence assav and compared with that of 20 healthy adults. IL-2 level in some ofthese patients and healthy adults was determined stimulatively;The results revealed that innormal controls the percentage of IL-2+cells in PBMC in unstimulated cuIture was 2.33±2.52%and 1.75±1.26%in patients with severe hepatitis;There was significant increase inIL-2+ cells stimulated by phytohemagglutin(PHA)after 48 hours,but the increase of percent-age of IL-2+ cells was significantly less in patients with chronic active hepatitis(35. 50 ±13.00%)and severe hepatitis (38. 08±14. 01%)than that of controls(60.55±13.79%;P<0.01), but there was also a considerable overlap. The peak of IL-2R expression was de-laved for 24 hours in patients with severe hepatitis. The IL-2 activity of 20 cases of severehepatitis was lower than that of 13 cases of healthy adults.The results indicate that the abilitv of IL-2R expression on PBMC in patiens with severehepatitis was impaired. There was IL-2 secretion defect in vitro, but there was no correlationbetween the IL-2R expression and level of IL-2 in patients with severe hepatitis. The mecha-nism of defect of IL-2R expression in patients with severe hepatitis might be sophiscated.