葡萄糖苯脎分光光度法测定血清糖化蛋白的初步研究

PRELIMINARY STUDY ON DETERMINATION OF SERUMGLYCATED PROTEINS USING GLUCOSE PHENYLOSAZONE SPECTROPHOTOMETRIC ASSAY

用葡萄糖苯脎分光光度法检测血清糖化蛋白。结果表明，此法线性范围宽（２５～１０００μｍｏｌ／Ｌ），精密度好（批内ＣＶ２．３％～３．３％，批间ＣＶ３．１％～４．５％），回收率高（９７．６％）。显色后室温下放置２ｈ稳定。多种成分如葡萄糖、半乳糖、胆红素、血红蛋白、甘油三酯在正常参考值范围内的干扰小于１０％。用此法测定３８名正常成人的血清糖化蛋白浓度，建立了正常参考值，并对其临床应用进行了初评。

Serum glycated proteins were determined by glucose pheny losazone spectrophotometricmethod.The resuIts showed that the above assay had a wide linearity(25～1000μmol/L),good precision(CV values of within一run 2.3%～3.3%,CV values of between一day 3.1%～4.5%)and high recovery rate(97.60%).The values of absorptance remained constant atroom temperature for 2 hours after coloration,The interferences of several components(glucose,galactose,bilirubin,hemoglobin,triglyceride),which were in their normal referenceranges,were less than 10%。The concentfrations of glycated proteins in the serum of thirty一eight heaIthy adults were detected and the normal reference values were established.The evaluation of its clinical applications is briefly discussed。