摘要
十二烷基硫酸钠──聚丙烯酰胺凝胶水平超薄层孔径梯度电泳，结合考玛斯蓝（或银）染色是一种操作简单，样品量微，分辨率及敏感性高且能快速分离测定尿蛋白分子量的方法。在一块凝胶板（１２０×２５０×０．５ｍｍ）上可同时分离２４个样品，样品不需浓缩，且只需４ｕｇ，分离长度为８ｃｍ，只需３ｈ～４ｈ即可获得结果。
Horizontal ultrathin-layer SDS pore gradient polyacrylamide gel
electrophoresis in com-bination with coopassie blue(or silver)staining was a highly
sensitive,accurate,and rapidmethod vvith nucro-sample for the separation and molecular weight
determination of urineproteins,The 24 samples could be separated in one gel(gel size l20mm×
250mm×0.5mm).The urine sample was ouly 4ug with 8cm separation distance,The results
were obtainedwithin 3-4 hours.
出处
《湖南医科大学学报》
CSCD
1994年第1期67-69,共3页
Bulletin of Hunan Medical University
关键词
电泳
聚丙烯酰胺
凝胶
蛋白尿
electrophoresis,palyacrylamide
gel
proteinuria
diagnosis use
method
