摘要
从国标法初发酵管中，吸取产醚产气的菌液，滴于滤纸上进行β－半乳糖苷酶及氧化酶检测。若试验的滤纸在长波段紫外线灯下发绿色荧光，而氧化酶试验为阴性的革兰氏阴性杆菌，则确定该初发酵管中存在大肠菌群菌，无须再作平板分离与复发酵。此两种酶试验所需细菌的最低浓度为２×１０６ＣＦＵ／ｍｌ及２×１０７ＣＦＵ／ｍｌ。此法与国家标准法相关良好（ｒ＝０．９５），在排除非本质误差之后，两法吻合率为１００％。
The bacteria solution was drown from the tubes
of first gas-forming lactose fermenta-tion(standard method),and dropped down on the filter
paper and then the experiment of β-galactosidas(β-GALA) and oxidase was made. If the
tested paper under a hand showed greenfluorescent light (366nm)While oxidase was negative ,
gramnegative rods,the coliforms wereconfirmed ,it was not necesary to have isolation and
second fermentation.The β-GALA testwas positive with a minimal concentration of 2 × 106
CFU/ml of coliforms and the oxidasetest was positive with 2 ×107 CFU/ml for aeromonas spp.
The rapid enzymatic method hadgood relativity to standard method,r=0. 95,agreement between
two methods was 100%,af-ter eliminting non-essential difference.
出处
《湖南医科大学学报》
CSCD
1994年第2期177-180,共4页
Bulletin of Hunan Medical University
关键词
肠杆菌科
半乳糖苷酶
氧化还原酶
Enterobacteriaceae
fruocescent agent
method
galactosidases
oxi-doreductases I thank James D. Berg
,Ph.D.(Norwegian water technology center)providing 4-MU-β-D-galactoside to make possible .
