人参总皂甙对苯诱发再障大鼠红细胞中四种已糖利用的影响

EFFECTS OF GINSENOSIDES ON THE UTILIZATION OF FOUR HEXOSES BY THE ERYTHROCYTES OF RATS WITH BENZENE INDUCED APLASTIC ANEMIA￣①

采用吸苯法建立大鼠实验性再障模型。观察到大鼠再障早期，红细胞内糖酵解加快，除半乳糖外，磷酸己糖旁路中甘露糖、葡萄糖和果糖的氧耗率减弱；再障后期，糖酵解逐渐变慢，己糖的氧耗率呈代偿性增强。人参总皂甙能明显改善再障大鼠外周血象和骨髓象，延缓红细胞乳酸的生成，并显著增加己糖的利用；其影响程度依次为：甘露糖＞葡萄糖＞果糖＞半乳糖。

Twenty four Wistar rats were divided into 3 groups at random:normal control(6 rats);aplastic anemia(AA; 12 rats)and AA treated with ginsenosides ip(6 rats;0 mg/kg bodyweight).AA models were established by subacute benzene inhalation within 8 wk.The RBC,WBC counts and hemoglobin(Hb)contents in their peripheral blood as well as the lactic acidlevels and the utilization rate of 4 hexoses in rat erythrocytes were observed before and afterbenzene inhalation and after one course of ginsenosides treatment.Four weeks after benzeneinhalation,RBC,WBC counts and Hb contents in the peripheral blood of AA rats showed atendency of significant decline。During the early period of benzene AA,the rate of glycolysisin the rat erythrocytes increased considerably. Excepting the galactose the intensity of theutilization of 3 other hexoses in their pentose phosphate pathways exhibited some degree ofdecrease. Having entered the late period of benzene AA,the glycolysis of rat erythrocytes wasslowed down gradually and a compensatory elevation in their oxygen consumption rate was seen. Ginsenosides improved the blood and bone marrow pictures of the AA rats,diminishedthe production of lactic acid in their erythrocytes and promoted distinctly their utilization of 4hexoses。 The order of their elevated rate of oxygen consupmtion was tnannose>glucose >fructose>galactose. The mechanism of pathobiochemical changes caused by the benzene AAand the therapeutic effects of ginsenosides were briefly discussed.Abstract was published on Blood,78(10)Suppl.408a,1991.②postgraduate③ preceptor④members ofadvisory group