不同基因型冬小麦花药出愈率及其愈伤组织的蛋白质电泳分析

Callus Inducing Frequency from the Anther of Different Genotypes Winter Wheat and the Electrophoretic Analysis of Proteins in Calli

用不同基因型的冬小麦品种（系）花药在Ｗ１４２培养基（改进的Ｗ１４培养基）上获得了较高的出愈率，但不同基因型之间仍存在着显著的差异。用Ｐｈａｒｍａｃｉａ公司ＰｈａｓｔＳｙｓｔｅｍ电泳仪对不同基因型小麦花药及其愈伤组织蛋白质进行等电聚焦电泳（ＩＥＦ）和ＳＤＳ─聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）分析，观察到不同基因型花药蛋白质之间存在着差异，并且初步认为这种蛋白质差异与出愈率有相关性。不同基因型花药愈伤组织之间观察不到明显的带型区别，但它们和花药比较，却明显有新蛋白质的产生，这可能与出愈过程生化变化有关。

The high frequencies of callus induction were obtained from anthers of different geontypes winter wheat on the W142 mediun(a modified W 14 medium).Different genotypes showed significant differences in the frequency of callus induction,IEF and SDS-PAGE analyses on the proteins of wheat anthers and calli thereof were performed with the Phast System Electrophoresis apparatus made by the Phamacia Company.Differences were observed in proteins from the anthers of different genotypes and relationship was considered to exist between the difference and the frequencies of callus induction.No obvious difference was observed in band patterns of anther calli among different genotypes.It was found that there some new protein bands in calli when compared with those of the anthers.This phenomenon probably resulted from the biochemical changes in the process of callus induction.