高产谷氨酸菌S9114的选育及生产实绩

BREEDING AND APPLICATION OF A STRAIN OF HIGH GLUTAMIC ACID YIELDING BACTERIUM S9114

以天津短杆菌（Ｂｒｅｖ．ｔｉａｎｊｉｎ）Ｔ６－１３出发，经紫外线、亚硝基胍用原生质体等诱变处理，选育出Ｓ９１１４菌株，具有耐高糖、耐高谷氨酸，不利用谷氨酸和产酸率高的特性。摇瓶产酸最高达９６．９ｇ／Ｌ，比原出发菌提高５０％。经工艺条件优化，在２０ｍ３发酵罐最高产酸９２．３ｇ／Ｌ（转化率５６．５％）。在６０ｍ３发酵罐连续１０批平均产酸８２．１ｇ／Ｌ，转化率４８．７４％、在１５００升发酵罐进行高生物素添加青霉素流加糖的工艺实验，最高产酸达９８．７ｇ／Ｌ，转化率５２．２％，周期２６小时。

A new strain of bacterium S9114, is bud from Brev. tianjin T_(6-13)by induced mutagenesis of the protoplasts with ultraviolet and NTG, etc.The strain S9114 shows the charaCteristic of enduring higher sucroseconcentration and higher glutamic acid concentration, non-glutamic acidconsumed and higher acid yield. The. highest glutandc add yield by shaking flask is up .to 96.99/L, 50% higher than that of the original strain.In 20m￣3 fermentor, the highest acid yield is 92.3g/L and the highest inversion rate is 56.5%. And in 60m￣3 fermentor, the average acid yield and inversion rate of ten batches of fermentation experiment are 82.1g/L and48.74%, especially under the optimum technological condition. The experiments of penicillin, high biotin and complementing sucrose technique areundergone in 1500L fermentor, in which the highest acid yield is 98.7g/Land the highest inversion rate is 52.2% afted a cycle of 26 hours.