液膜法提取发酵液中的苯丙氨酸Ⅰ．膜的配方及其稳定性

Extraction of Phenylalanine from Fermentation Broth by Liquid Emulsion Membrane (LEM)Ⅰ.Composition and Stability of LEM

采用乳化液膜法，对从发酵液中分离和浓缩苯丙氨酸（Ｐｈｅ）的液膜配方和稳定性进行研究。得出了既能保持较好的膜稳定性又能保持较高分离效率的膜组成：载体二（２－乙基已基）磷酸５％，表面活性剂（ＥＭ－３０１）５％，膜溶剂（工业煤油）９０％。内相试剂选用１．６ｍｏｌ／Ｌ的ＨＣｌ溶液。采用高强度（＞３２００ｒ／ｍｉｎ）制乳，能够有效地控制溶胀和破损率。对Ｐｈｅ的萃取结果表明：８０％以上的Ｐｈｅ能够从外相浓缩到内相，最后内相可达４～５倍的浓缩率。

The membrane composition with 5%di(2-ethylhexyl)phosphoric acid as the canier,5%EM-301 as the emulsifier and 90%kerosene as the solvent was proved to be the most effective. HCl with a concentration of 1.6mol/L was used as the internal phase. The breakage and swell of the emulsion can be efficiently limited under the condition of a strong emulsification agitation speed (>3200r/min).It has been demonstrated that more than 80% of phenylalanine can be extracted from the external phase and the final phenylalanine concen-tration in internal phase is 4～5 times as high as the initial concentration in the external phase.