用于细胞内自由［Ca^（2＋）］测定的新萤光试剂Fura－2AM的合成、性能及应用研究

Studies on Synthesis Properties and Application ──the New Fluorescent Reagent Fura-2 AM For Cytosolic [Ca￣(2+)]

用于细胞内自由［Ｃａ２＋］生理作用规律研究的新强萤光试剂Ｆｕｒａ－２ＡＭ已由十七步合成成功。与过去广泛使用的该类试剂相比，其萤光强度高几十倍，对［Ｃａ２＋］亲和力稍弱，对镁和其它二价离子的选择性更好，特别是结合［Ｃａ２＋］后波长发生移动。这些优点使它成为目前最重要的特别是测定单细胞中［Ｃａ２＋］的萤光试剂之一。

A new highly fluorescent reagent Fura-2 AM has been synthesized by 17 steps for study on physiological rule of cytosolic free Ca2+. Compared to their widely useful prodecessor, it shows much stronger fluorescence, wavelength shifts upon Ca2+ binding, somewhat weaker attinity for Ca2+ and better selectivity against magnesium and other divalent ions. These advantages make it one of the important fluorescent reagent for [Ca2+] especially in single cells.