利用小麦5B效应引入外源基因的研究──（Ⅰ）易位系的培育

Transfer of Nonwaxy Gene from Elytriqia elonqata Using 5B Effect of Wheat──(Ⅰ) Breeding Procedures

ｄｕｒｕｍ小麦的代换系ｄｉ－ｓｕｂ５Ｄ（５Ｂ）与添加系ｄｉ－ａｄｄｅ４ｔｓ杂交，再用ｄｉ－ｓｕｂ５Ｄ（５Ｂ）进行回交。在自交后代中选育出了易位系１０３２。该易位系染色体数２ｎ＝２８，表现型为非蜡质（ｎｏｎｗａｘｙ）。这一结果证明厂在ｄｕｒｕｍ小麦中也可以利用５Ｂ染色体效应。通过诱发部份同源染色体间的配对，获得易位体。

The ditelosomic addition of the short arm of Elytrigia elongata chromosome e4, located a wax inhibitor gene (WI) to Triticum durum L. cv. Stewart, was crossed with the disomic substitution SD(5B) of durum wheat. Then the F1-plants were back-crossed by the disomic substitution 5D(5B). Among the selfed progeny a plant was selected which had 28 chromosomes and showed nonwaxy phenotype. The line 1032 was obtained as selfed progenies of this plant.Our results suggest that: (1) homoeologous pairing is promoted when it is short of 5B chromosome; and (2) recombination can induce to transfer the nonwaxy phenotype from chomosome e4ts to its homoeologous durum wheat chromosome.