荧光光度法测定饲料中微量硒的探讨

A STUDY ON USING THE SPECTROFLUOROMETRICMETHOD FOR DETERMNING TRACE SELENIMIN FODDER

本文在前人研究的基础上对荧光法测定微量硒作了进一步的改进与简化，结果表明，操作简便易行。硒与２，３－二氨基萘在酸性条件及适当的温度下，形成络合物。用ＥＤＴＡ掩蔽干扰离子，以环已烷萃取，取其有机相直接在Ｈ１ＴＡＣＨＩ６５０—１０ＬＣ荧光检测器５５５ｍｎ处（激发３７４ｎｍ）测荧光强度。避光保存时络合物至少稳定２４ｈ。相对标准差在３％～５％之间。

The method of spectrofluorometric determination of trace Se was improved and sim-plified based on the earlier studies. Under conditions of appropriate acidity andtemperature, the fluorescent Se 2,3 diaminonaphthalene complex was formed bychelation in the presence of EDTA and then separated from interfering fluorescentspecies by extraction with cyclohexane.The intensity of its fluorescent emission canbe measured directly at 555 nm(excitation at 374 nm)by the HITACHI 650 10LCFluorescenCe Spectrophotometer.The relative standard deviation was 3 %～5%.