一阶导数光谱法测定聚α－氰基丙烯酸正丁酯毫微粒中硫酸庆大霉素的包封率

Determination of Associating Ratio of Gentamycin Sulfate in Polybutylcyanoacrylate Nanoparticles by First Derivative Spectrum

作者通过低温超速离心法分离硫酸庆大霉素聚α－氰基丙烯酸正丁酯毫微粒胶体溶液的上清液，用电荷转移络合物－一阶导数光谱法测定其中硫酸庆大霉素的含量，并计算其包封率。结果表明：该法回收率为１０１．１５％，变异系数为１．７０％，在０．４～２０ｕｇ／ｍｌ范围内线性关系良好，较微生物测定法简便、快速，可靠。用该法测定硫酸庆大霉素毫微粒的包封率，可作为其处方筛选和质量控制的手段。

The associating ratio of gentamycinsulfate in polybutylcyanoacrylate nanoparticles was de-terinatated by separating the supermnant firstlythrough the centri-fugation(4℃,34 000 r/min,2. 5h),formina the charge-transfer complex using chloranil,and then estimating the drug content in supernatant byfirst dervative spectrum. The polybutylcyanoacrylate insupernatant was proved to be not interfering.The re-covery of drug from supernatant was 101.15%,andthe coefficient of variation 1.70%.