放射性生物微球技术测定狗下颌骨区域血流量

Measurement of Regional Bone Blood Flow in the Dog's Mandible Using the Radioactive Microsphere Technique

分离蟾蜍红细胞，用５１Ｃｒ标记之，然后经福尔马林固定，制成不易破碎的，没有免疫源性的放射性生物微球。对这种微球的理化性质进行了鉴定，并用其测定了幼年狗下颌骨的区域血流量。结果表明，放射性生物微球在理化性质方面完全达到了微球技术的要求，其大小非常一致，当被用于组织血流量测定时，放射性生物微球相当于１５μｍ的成品微球。这种微球通过左心房输入到体循环后，对实验动物的循环生理无明显的干扰作用。微球在体内与血液非常均匀地混合，根据血流量大小按比例分布到全身各组织器官，并在第一次循环中全部嵌塞于毛细血管内。放射性生物微球完全可以替代国外的成品碳化微球而用于下颌骨区域血流量的测定。

Abstract Regional bone blood flow in the dog's mandible was measured by using the radioactive microsphere technique(RMT).Toad red blood cells were labelled with 51Cr,fixed in 10%formalin,and thus became a kind of microspheres whose density was quite similar to that of the dog's red blood cells.It was found that this kind of radioactive microspheres are fully up to the standards of microsphere technique,Their sizes are extremely uniform, and when used to measure the tissue blood flow,they serve as 15 μm carbonized microspheres.After being injected into systemic circulation through left atrial catheter, these microspheres have no distinct influences on physiological circulation of the animal.Microspheres were very evenly mixed with blood in the body, distributed to its every part proportionally to the blood flow rates,and were completely trapped in the capillaries and arterioles during the first circulation,The anthors thus suggest that 51Cr labelled toad red blood cells are an ideal marker for the measurement of regional bone blood flowin the dog's mandible.