唾液对变形链球菌Mutans和变形链球菌Sobrinus粘附的影响

The Effect of Saliva on Adherence of S.mutans and S.sobrinus

用￣３Ｈ标记变形链球菌遗传分型Ｉ型Ｓ．ｍｕｔａｎｓＪＢＰ和Ⅲ型Ｓ．ｓｏｂｒｉｎｕｓ６７１５，观察细菌在经兔唾液包被的羟磷灰石（ＨＡ）微珠表面的粘附量，以研究唾液对两种菌粘附的影响。结果表明，Ｓ．ｍｕｔａｎｓＪＢＰ和Ｓ．ｓｏｂｒｉｎｕｓ６７１５对经唾液包被的ＨＡ上的粘附量有明显差异，Ｉ型ＪＢＰ的粘附量显著多于Ⅲ型６７１５（Ｐ＜０．０１），在无唾液包被的ＨＡ上，两者粘附量无明显差异（Ｐ＞０．０５）。结果说明变形链球菌Ｉ型Ｓ．ｍｕｔａｎｓ粘附的条件，在口腔唾液环境易于满足，从而解释Ｓ．ｍｕｔａｎｓ在流行病学调查中的优势地位。

Abstract An adhesive study was tested with S.mutans and S.sobrinus radiolabeled by[3H].The adsorptive behavior of S.mutans JBP and S.sobrinus 6715 to pellicles formed from saliva of ribbit on hydroxyapatite beads was abserved.The results showed that the saliva pellicles markly enhanced attchment of S.mutans but not enhanced attchment of S.sobrinus(P<0.01)and both organisms showed no difference on HA beads without saliva pellicles(P>0.05).It seems that the saliva could satisfy some conditions for the adherence of S.mutans JBP and this may be one reason to explain the dominance of S.mutans in epidemiolgical investigation.