正常人肝细胞表面人多聚白蛋白受体的放射分析

Radioligand Binding Assay of PHSA Receptor on Hepatocytes

作者用受体放射分析法在７例正常成人肝细胞上检测到人多聚血清白蛋白（ＰＨＳＡ）受体的存在，受体的亲和常数（Ｋｄｓ）及结合容量（Ｃ）存在个体差异，其均值分别为：Ｋｄ＿１＝９．６７６９±５．２７６７ｆｍ￣（－２），Ｃ＿１＝８６．１４６８±４６．４１２９ｆｍ／μｇ；Ｋｄ＿２＝１０３．１８１０±５６．４７７０ｆｍ￣（－２），Ｃ＿２＝２６１．５３０２±１７５．９０９４ｆｍ／μｇ。Ｓｃａｔｃｈａｒｄ作图为一曲线，提示肝细胞上的ＰＨＳＡ受体由两种不同亲和力及结合容量的结合位点组成。本文还对该受体在ＨＢＶ嗜肝机理中可能的作用及其意义进行了讨论。

The radioligand binding assay of PHSAreceptor on hepatocytes of 7 adults is reported.Thepresence of PHSA receptor was demonstrated in all the7adluts, though the individual difference of the Kdsand binding capacities among them was distinct with ahigher standard derivation.The mean values of theKds and binding capacities were as follows:Kd_1=9. 6769±5.2767fm￣2,C_1=86. 1468±46. 4129fm/μg;Kd_2 = 103. 1810±56. 4770fm￣(-2), C_2=261. 5302±175. 9094fm/μg, The Scatchard plot was a curve whichindicated the PHSA receptor on the hepatocyte wascomposed of two binding sites of different affinitiesand capacities, The possible mechanism and signifi-cance of PHSA receptor in the hepatotrophism are alsodiscussed.