乳腺癌组织及血清同工铁蛋白水平的观察

Detection of Isoferritin Level in Breast Cancer Tissue and Serum

利用酶联免疫吸附法（ＥＬＩＳＡ）检测了２５例人乳腺癌组织（ＢＣＴ）、６例癌旁组织（ＣＳＴ）及８例正常乳腺组织（ＮＢＴ）的胞浆提取液同工铁蛋白（ＣＩＦ）水平，结果分别为１６０２３±１５７３２、２４６７±１３６０及１３８２±６４７ｎｇ／ｍｃｐ，ＢＣＴ及ＣＳＴ的ＣＩＦ浓度分别为ＮＢＴ的１１．６及１．８倍。（Ｐ＜０．００１及Ｐ＜０．０５）．同时检测了９６例正常健康人，６８例乳腺癌病人的血清同工铁蛋白（ＳＩＦ）水平，结果分别为５８．７±３５．８及４７３．３±２９７．３ｎｇ／ｍｌ，癌症患者约为正常人的８．１倍（Ｐ＜０．００１）。研究结果表明ＢＣＴ的ＣＩＦ水平是乳腺组织癌变的重要参数；ＢＣＴ的ＣＩＦ可能是ＳＩＦ升高的来源，由此启示ＳＩＦ可考虑作为乳腺癌的早期诊断指标。

ELISA(double-determinant)was usedto evaluate the isoferritin level in the breast cancer tis -sue. The isoferritin concentration was measurd in cy-tosol extracts of 25 breast cancer tissues(BCT).6cancer surrounding tissues(CAT)and 8 normal breasttissues (NBT).With the results of 16 023±15 732,2467±1360 and 1382± 647ng/mcp(milligrams of on-tosol protein),respectively. The isoferritin concentra-tions of BCT and CST were 11.6 and 1. 8 times asmuch as that of NBT.and the differences were statis-tically significant(P<0. 001 and0. 05).The serumisoferritin level vvas also measured in 96 healthy per-sons and 68 breast cancer patients (mean 58.7±35.8and 473. 3±297. 3ng/ml) with the difference as big as8. 1 times.The results have demonstrated that thelevel of cytosol isoferritin in BCT is an important pa-rameter of breast tissue malignance and may be alsoresponsible for the serum isoferritin increase. There-fore we think that serum isoferritin may be used as amarker for early diagnosis of breast cancer.