脑挫伤区及其远离部位脑组织中纤维蛋白的免疫组化观察

Immunohistochemical Study on Fibrin in Brain Traumas

作者应用免疫组化ＰＡＰ法对诊断确切，死亡时间明确的１１例脑挫伤标本，包括挫伤局部及远离部位脑组织进行染色观察，结果发现：所有脑挫伤局部均有纤维蛋白形成；在挫伤远离部位、脑基底部、小脑、脑干等处的组织细胞和部分血管内及血管周围亦有纤维蛋白形成；在整个暴力传导通路上的脑组织细胞均有损伤。因此，作者认为脑挫伤应视为脑弥漫性损伤，不宜仅视为局部损伤。在脑组织细胞及血管内观察到纤维蛋白可作为脑挫伤的诊断指标之一。对单独存在的脑干损伤，用ＨＥ染色及纤维蛋白的免疫组化染色对比观察，可明显提高其诊断率。

The fibrin formation in brain traumaswas studied by immunohistochemical method.Elevencases of brain trauma were examined.The fibrin wasdemonstrated not only in the areas of brain contusionand the neuronal cytoplasm of hypothalamus,thala-mus,brainstem , and cerebellum,but also on themembrane of endothelial cells and red blood cells with-in some capillaries.The fibrin was also found aroundcapillaries and the areas far from the brain trauma. Itwas suggested that injuries to the brain occurred notonly in the Iocal areas of the brain subjected to the vio-lence,but also in the areas far from the local injury. Itmeant that the brain injuries were not local,but dif-fuse. The fibrin observed in the neuronal cytoplasm,and on the membrane of endothelial cells and red bloodcells vvithin some capillaries was a sign of ante mortembrain traumas.The combined HE staining and im-munohistochemical staining of the fibrin is useful inthe demonstration of simple brainstem traumas.