摘要
用免疫球蛋白重链J区(JH),K轻链及λ轻链C区(Ck、Cλ_2)基因探针及Southern印迹杂交技术,对22例非何杰金氏淋巴瘤进行分析。结果显示13例B细胞淋巴瘤均出现重链基因重排,其中10例行K或λ轻链基因重排;3例T细胞淋巴瘤中,1例出现重链,每1例出现K轻链基因重排。1例分类未明淋巴瘤出现重链基因重排。5例未作免疫表型分析的淋巴瘤中有4例出现重链,3例出现K轻涟,1例出现λ轻链基因重排。对免疫球蛋白基因重排分析在淋巴瘤诊断中的意义进行了讨论。
The B-cell clonality of 22 cases of non-Hodgkin's lymphoma was analysed by using the immunoglobulin JH, CK and C_λ gene probes and Southern blot hybridization method.All the 13 cases of B-cell lymphoma, which were confirmed by histopathology and immunohistochemistry, showed the non-germlime rearrangement of Ig heavy chain gene, 10 of them being with k or λ light gene rearrangement, whereas one of the three T-cell lymphomas with Ig heavy chain gene and another one with k light chain gene rearrangement. One case of undetermined lymphoma was with heavy chain gene rearrangement. In the other 5 cases of lymphoma, which were without immunophenotyping, there were 4 heavy chain, 3 with k light chain and one with λ chain gene rearrangements. The results suggest that Ig gene rearrangement, especially when the heavy and light chain gene rearrangements are found in the same cae, is the specific molecular biological marker of B-cell lymphoma.
出处
《华西医学》
CAS
北大核心
1994年第2期170-173,共4页
West China Medical Journal
基金
四川省科委应用基础研究基金
关键词
基因重排
淋巴瘤
免疫球蛋白
Gene rearrangement
B-cell lymphoma
Southern blot hybridization