免疫球蛋白基因重排分析在淋巴瘤诊断中的初步应用研究

Preliminary Study of Immunoglobulin Gene Rearraugement Analysis to Lymphoma Diagnosis

用免疫球蛋白重链Ｊ区（ＪＨ），Ｋ轻链及λ轻链Ｃ区（Ｃｋ、Ｃλ_２）基因探针及Ｓｏｕｔｈｅｒｎ印迹杂交技术，对２２例非何杰金氏淋巴瘤进行分析。结果显示１３例Ｂ细胞淋巴瘤均出现重链基因重排，其中１０例行Ｋ或λ轻链基因重排；３例Ｔ细胞淋巴瘤中，１例出现重链，每１例出现Ｋ轻链基因重排。１例分类未明淋巴瘤出现重链基因重排。５例未作免疫表型分析的淋巴瘤中有４例出现重链，３例出现Ｋ轻涟，１例出现λ轻链基因重排。对免疫球蛋白基因重排分析在淋巴瘤诊断中的意义进行了讨论。

The B-cell clonality of 22 cases of non-Hodgkin's lymphoma was analysed by using the immunoglobulin JH, CK and C_λ gene probes and Southern blot hybridization method.All the 13 cases of B-cell lymphoma, which were confirmed by histopathology and immunohistochemistry, showed the non-germlime rearrangement of Ig heavy chain gene, 10 of them being with k or λ light gene rearrangement, whereas one of the three T-cell lymphomas with Ig heavy chain gene and another one with k light chain gene rearrangement. One case of undetermined lymphoma was with heavy chain gene rearrangement. In the other 5 cases of lymphoma, which were without immunophenotyping, there were 4 heavy chain, 3 with k light chain and one with λ chain gene rearrangements. The results suggest that Ig gene rearrangement, especially when the heavy and light chain gene rearrangements are found in the same cae, is the specific molecular biological marker of B-cell lymphoma.