ABC免疫电泳对膀胱癌P_（21）蛋白表达的观察

Detection of Ras Oncogene Product-P21 Protein in Human Bladder Transitional Cell Carcinoma with ABC Immunoelectrophoresis

用ＡＢＣ免疫电泳检查了２９例膀胱移行细胞癌、６例癌旁粘膜、３例膀胱炎和３例正常膀胱粘膜的Ｐ_（２１）蛋白表达，发现２５例（８６．２％）膀胱癌、３例癌旁组织、１例正常膀胱粘膜、２例膀胱炎组织有Ｐ_（２１）蛋白表达。随肿瘤分级增高，Ｐ_（２１）蛋白条带的光密度积分增加；肿瘤组织Ｐ_（２１）蛋白光密度积分高于癌旁组织，癌旁组织又高于正常膀胱粘膜。

The expression of P_(21) was detected in 41 samples of bladder tissue with ABC immunoelectrophresis. The positive rate of P_(21) expression was 86.2% (25/29) in bladder transitional cell carcinoma, 50% (3/6) in para-carcinoma tissue,33. 3% (1/3) in normal bladder mucosa and 66. 7%( 2/3) in bladder inflammatory mucosa.The immunoelectrophoretic film computing densitometer intergrated area was 1530,1938,3605 in gradeⅠ,Ⅱ, Ⅲ of bladder carcinoma respectively.The average integrated area of immunoelectrphoretic P_(21) band was 2595 in bladder carcinoma tissue,963 in para-carcinoma, 682 in normal mucosa, 1678 in bladder inflammatory mucosa.