肺癌病人手术前后T淋巴细胞亚群的动态研究

Pre- and Postoperative Dynamic Study on the T Lymphocyte Subsets of the Peripheral Blood in Patients with Lung Cancer

本实验应用ＯＫＴ单克隆抗体以及免疫荧光技术，对５８例肺癌，２４例良性胸部疾病病人进行手术前后外周血Ｔ淋巴细胞亚群的动态检测，并以５２例健康人对照。本实验观察到肺癌病人的比值明显降低，明显增高。上述变化与肺癌的病期、组织学类型有密切关系。肺癌根治术后１周内，免疫抑制进一步加重，２周后逐渐恢复，术后４周可达正常水平。本实验结果表明：肺癌病人有明显的细胞免疫功能障碍和免疫抑制存在，手术切除肺癌可解除宿主的免疫抑制，使病人的免疫平衡得以恢复。

A serial changes of T lymphocyte subsets of the peripheral blood were detected by indirect immunofluorescent assay with mouse antihuman monoclonal antibody of OKT3,OKT4 and OKT8 in 58 patients with lung cancer before and after operation. In comparising theee tests with those of 24 casee of noncancerous thoracic lesions and 52 normal adults, the number of OKT3, OKT4 and OKT4/OKT 8 ratio from the cancer patients was significantly lower than that of the noncancerous thoracic lesions and normal individuals. On the contrary, the OKT8 cell from cancer patients was remarkably higher than that of the noncancerous thoracic lesions and normal adults. The degree of these changes was closely related to the stagcs of cancer and type of histological classification. The immunosuppression existing before operation became more severe within 1 week after the removal of the tumor and gradually recovered on the 14th postoperative day. It returned to normal level about 4 weeks postoporatively. These results suggested that patients with lung cancer had remarkable immunosuppression and impairement of cellinduced immunological function; and that surgical operatioh not only removed the tumor,but also broke the vicious circle of immunosuppression. Th erefore, the immune balance of patients might recovery after operation.