黑木耳多糖抗肿瘤作用的实验研究

STUDIES ON ANTITUMOR ACTION OF POLYSACCHARIDES FROM AURICULARIA AURICULA

用１８～２０ｇ的昆明系小白鼠研究水溶性、碱溶性及碱不溶性三种黑木耳多糖对移植性肉瘤－１８０的抗肿瘤活性。水溶性和碱溶性黑木耳多糖按５０ｍｇ／ｋｇ和２００ｍｇ／ｋｇ分别分为两个剂量组及一个对照组，每组实验动物数为１０只；碱不溶性黑木耳多糖按１０ｍｇ／ｋｇ和３０ｍｇ／ｋｇ分为两个剂量组及一个对照组，每组实验动物６只。每天经腹腔注射一次，连续１０天，停药一周后检查肿瘤抑制率。结果表明：水溶性黑木耳多糖注射剂量５０ｍｇ／ｋｇ和２００ｍｇ／ｋｇ对肉瘤－１８０的抑制率分别为４４％和８９％。碱溶性黑木耳多糖注射剂量２００ｍｇ／ｋｇ对肉瘤－１８０的抑制率为３１％。碱溶性黑木耳多糖注射剂量５０ｍｇ／ｋｇ及碱不溶性黑木耳多糖注射剂量分别为１０ｍｇ／ｋｇ、３０ｍｇ／ｋｇ对肉瘤－１８０均无明显抑制作用。

The tumor-inhibitory activities of polysaccharides from Auricularia auricula were compared by successive, intraperitoneal administration of each kind of polysaccharide for 10 days, starting 24h after subcutaneous implantation of sarcoma 180 cells in kunming strain mice.The mice were divided randomly into test and control groups(every group with 6～10 mice). One week after ceasing administration, tumor inhibition ratios were examined.The results exhibited that the water-soluble polysac charide at the dosage of 50 and 200 mg/kg body wt per day showed significantly inhibitory effect(P<0. 01), and the tumor-inhibition ratios were 44%and 89%respectively.The alkali-soluble polysaccharide at a dosage of 200 mg/kg body wt per day showed significantly inhibitory effect (P<0. 05), with a tumor-inhibition ratio of 31%.The alkali-soluble polysaccharide at a dosage of 50 mg/kg per day and the alkali-insoluble polysaccharide at a dosage of 10～30 mg/kg body wt per day showed essentially no inhibitory effect(P>0.05).