番茄子叶离体培养与再生成株

EFFICIENT TOMATO PLANT REGENERATION USING COTYLEDON EXPLANTS CULTURED IN VITRO

取番茄不同苗龄的无菌苗子叶进行培养，发现以１４天苗龄的子叶培养不定芽再生频率最高，在ＭＳ培养基上添加不同浓度的吲哚乙酸（ＩＡＡ）和玉米素（Ｚｔ），对子叶愈伤组织的形成影响不大，但对不定芽的分化有较大影响，其中以ＭＳ＋Ｚｔ１ｍｇ·Ｌ^（－１）＋ＩＡＡ１ｍｇ·Ｌ^（－１）效果最好。在同样的培养基上继代培养，待芽长至１～２ｃｍ长时转到生根培养基上即可在短期内长出根系，成为再生植株。

The cotyledons of axenic seedlings of tomato(Lycopersicon esculentum Mill.)were cultured in vitro.Using 14-day old cotyledons with petioles as explants and a combination of Zeatin and IAA in MS basal media,about 90% of the explants produced shoots in 2 weeks.The optimal conditions for regeneration were found to include a zeatin concentration of 1 mg.L-1 and IAA concentration of 1 mg.L-1.The roots developed from shoots.in the rooting medium and the regenerants were transferred into pots for further growth.