紫云英根瘤菌7653R基因文库的5个互补结瘤菌株的重组质粒分析

THE ANALYSIS OF FIVE RECOMBINANT PLASMIDS FROM COMPLEMENTAL NODULATION STRAINS OF GENE LIBRARY OF RHIZOBIUM HUAXUII STRAIN 7653R

对紫云英根瘤菌７６５３Ｒ基因文库的５个重组质粒，即ｐＮＲ１０２，ｐＮＲ１０３，ｐＮＲ１０８，ｐＮＲ２０３，ｐＮＲ２１３进行各种内切酶的酶切分析。结果证实它们均含有１．７ｋｂ的ＥｃｏＲⅠ－Ｂｇ１Ⅱ双酶切片段和１．９ｋｂ的ＥｃｏＲⅠ－ＳａｃⅠ双酶切片段，而ｐＮＲ２１３的外源片段最大，包含有其余质粒所具有的各种片段。以ｐＮＲ１０８的外源片段作探针，进行ＤＩＧ杂交，发现探针与其余４个重组质粒的外源片段有强的同源杂交；与７６５３Ｒ的内源质粒杂交还表明，ｐＮＲ１０８的外源片段来源于共生质粒ｐＲａ７－２。

The recombinant plasmids, pNR102, pNR103, pNR108, pNR203,and pNR213, from Rhizobium huakuii strain 7653R (field type), were digested with differrent endonucleases. The cleavage mapping showed that they all contained 1.7 kb and 1.9 kb fragments. The probe was prepared with the foreign fragment of plasmid pNR108.Its DIG hybridization showed that the probe had extensive homologization with other foreign fragments of the recombinant plasmids. It also showed that the foreign fragment of plasmid pNR108 came from the symbiotic plasmid of strain 7653R.