摘要
应用色谱分离理论,综合考虑了分子扩散、进样量、电能消耗、电泳迁移等因素对高效毛细管电泳分离的影响,建立了高效毛细管电泳分离机理模型.以色氨酸为示踪样品进行实验,研究了电压、溶液浓度、进样量、毛细管管径等因素对理论板高度的影响,并与模型计算结果进行了比较.结果表明,本文所提出的模型能够较好地描述高效毛细管电泳的分离机理,该模型可作为优化操作参数和进行放大设计研究的基础.
The separation mechanism of high performance capillary electrophoresis was studied in light of chromatographic separation principle and by considering the effects of molecular diffusion, injection and power dissipation. The expression obtained for the height equivalent of a theoretical plate was compared with the experimental results. It was showed that the calculated results using the model were consistent with the experimental data. The model may provide the fundamentals for optimizing experimental conditions and scale up.
出处
《化工学报》
EI
CAS
CSCD
北大核心
1994年第4期475-481,共7页
CIESC Journal
关键词
毛细管电泳
生物产物
分离
机理
high performance capillary electrophoresis, bioproduct separation, separation technique
