环境中基因工程微生物的监测方法

Monitoring Methods for Genetically Engineered Microorganisms in the Environment

介绍国外有关环境基因工程微生物的监测原理与方法。即环境ＧＥＭ监测标记系统的基本要求；几种环境ＧＥＭ监测常用的标记系统；抗性标记系统、发色标记系统和发光基因标记系统等；环境ＧＥＭ的检测方法；平皿培养计数法、免疫学法、遗传学法、生物发光法和流动细胞计数器对环境ＧＥＭ监测。

his paper deals with the monitoring principle andmethods for genetically engineered microorganisms(GEM) in the environment, including therequirements of marker system on GEM formonitoring purpose, some recently developed markersystems and the monitoring methods forenvironmental GEM culture method, immunologicalmethod, genetic method, bioluminescent methodand application of flow cytometry.