SDS－PAGE和HPLC－荧光检测分离鉴定恩施高硒区大豆中含硒蛋白

SDS-PAGE Seperation and HPLC- FID Identification of Selenoproteins in Soybean from Enshi Area Having a Higher Level of Selenium in Soil

采用凝胶电泳技术分离恩施高硒地区大豆中的含硒蛋白组分，分辨出２７条蛋白条带。用高效液相色谱－荧光测定法对这些条带分别进行硒含量测定并进行空白扣除，发现其中有１３条带含硒。用标准分子量蛋白标定含硒蛋白组分分子量分别为：５８．１－６０．３；５２．５－５３．７；４６．８－５０．１；２９．５－３０．９；２８．８；２５．１－２５．７；２４．３；１９．７－２０．９；１８．４－１８．６；１６．８－１７．９；１６．１－１６．２；１５．２－１５．８和１４．３－１４．８ｋＤａ。

Selenoproteins in soybean from Ensh area where selenium in soil was at a higher level were seperated by using sodium dodecyl sulfste-polyacrylamide gel electrophoresis (SDS-PAGE) and then identified by using high Performance liquid chromatography with a fluorescent indication detector (HPLC-FID). Of 27 protein or protein subunit bands identified, 13were found to be selenium species. According to a standard protein kit, their molecular weights were estimated at 58. 3- 60. 3, 52. 5- 53. 7, 46.8- 50. 1,29. 5-30. 9, 28. 8, 25. 1- 25. 7, 24. 3,19. 7-20. 9,18. 4-18. 6, 16. 8- 17. 9, 16. 1-16.2, 15. 2-15. 8 and 14. 3- 14. 8 KDa,respectively.